Screening of process parameters for CO2 mitigation by using mutant Syhechoccus sp. PCC 7002

Mustafa, Iswaibah and Azmi, Azlin Suhaida and Jimat, Dzun Noraini and Amid, Azura and Ahmad Raus, Raha and Muyibi, Suleyman Aremu and Halmi Shari, Ummi and Zainal, Mus'ab (2013) Screening of process parameters for CO2 mitigation by using mutant Syhechoccus sp. PCC 7002. In: National Conference of Industry-Academia Initiatives in Biotechnology (CIA-Biotech13), 5th-7th Dec. 2013, Hotel Equatorial, Cameron Highlands, Pahang.

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Abstract

An increasing carbon dioxide (CO2) into atmosphere has led to unbalanced production of CO2 globally. Microalgae biofixation of CO2 by its photosynthesis metabolism process is an encouraging strategy for CO2 mitigation. Synechococcus sp. is an abundant unicellular microalgae and a major participant in global carbon cycles. Due to that reasons, this study was designed to screen the parameters that affecting CO2 fixation into cellular biomass via photosynthesis of mutant Synechococcus sp. (PCC 7002). The effects of four factors; light intensity, temperature, nitrogen-phosphorus-potassium (NPK) and NaNO3 concentration were studied by using Design Expert software version 8.0. The selected experimental design was Min-Run Res IV which consisted of 12 experimental runs. All of the experiments were conducted in shake flasks. Approximately, 15% (±1) of CO2 was supplied with constant flow rate. From the study, CO2 uptake and carbohydrate production were highest at temperature of 30ºC, light intensity of 2500 Lux, NaNO3 concentration of 1 % (w/v) and NPK concentration of 0 % (w/v). In this study, NPK did not give positive significant response to the microalgal growth. Since Synechococcus sp. is a blue-green microalga, it was found that the carbohydrate content was very low.

Item Type:	Conference or Workshop Item (Lecture)
Additional Information:	5339/34964
Uncontrolled Keywords:	microalgae, carbon dioxide mitigation
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Mrs. Azlin Suhaida Azmi
Date Deposited:	29 Jan 2014 12:40
Last Modified:	12 May 2016 13:46
URI:	http://irep.iium.edu.my/id/eprint/34964

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