Understanding thermostability factors of Aspergillus niger PhyA phytase: a molecular dynamics study

Noorbatcha, Ibrahim Ali and Sultan, Anas Mufid Nasri and Mohd. Salleh, Hamzah and Amid, Azura (2013) Understanding thermostability factors of Aspergillus niger PhyA phytase: a molecular dynamics study. Protein Journal, 32. pp. 309-316. ISSN 1572-3887

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Abstract

Abstract Molecular dynamics simulation was used to study the dynamic differences between native Aspergillus niger PhyA phytase and a mutant with 20 % greater thermostability. Atomic root mean square deviation, radius of gyration, and number of hydrogen bonds and salt bridges are examined to determine thermostability factors. The results suggest that, among secondary structure elements, loops have the most impact on the thermal stability of A. niger phytase. In addition, the location rather than the number of hydrogen bonds is found to have an important contribution to thermostability. The results also show that salt bridges may have stabilizing or destabilizing effect on the enzyme and influence its thermostability accordingly.

Item Type:	Article (Journal)
Additional Information:	3704/30144
Uncontrolled Keywords:	Aspergillus niger phytase � Molecular dynamics simulation � Thermostability � RMSD � Hydrogen bonds � Salt bridges
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof. Dr. Ibrahim Ali Noorbatcha
Date Deposited:	13 Jan 2014 08:54
Last Modified:	14 Jul 2021 17:27
URI:	http://irep.iium.edu.my/id/eprint/30144

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