Fabrication of micropillar sheet for cell culture dish

Ali, Mohammad Yeakub and Mohd Fuad, Nurul Hajar (2011) Fabrication of micropillar sheet for cell culture dish. In: Malaysia Technology Expo (MTE 2011), 17-19 February 2011, KLCC.

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Abstract

Cell culture is one of the major tools in life sciences. Conventionally, cells are placed in a flat petri dish made of glass or plastic. It is used to monitor cells behavior under controlled conditions. As the cells are strongly adhered to petri dish, an additional process of trypsinization is applied to remove the cells from the dish. Alternatively, by using micropillar sheet, the contact surface between the cells and the pillars is smaller than traditional two dimensional petri dish. Cells were found to grow in a different way on the micropillars because of these differences in adherence to the support materials. The micropillars structure enables the cells to be removed without any additional process where the cultured cells can be collected simply by pipetting. However, it remains a challenge to fabricate high aspect ratio metallic or polymeric micropillar sheets of about 1 μm or less of the micropillar diameters. This research presents the fabrication process using focused ion beam (FIB) a maskless sputtering technique for the fabrication of micropillar sheet to be used in cell culture dish. Micropore sheet on metallic materials is also machined using FIB sputtering which can be used as a master microtool for the mass production of polymer micropillar sheets by micro/nano hot embossing. The typical diameter of the micropillars is 1 μm with 3-10 aspect ratio. This FIB based process is direct, easy to fabricate, and less expensive compared to LIGA (lithography, electroplating and molding) and lithography based techniques as reported.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	4751/28907
Subjects:	T Technology > TJ Mechanical engineering and machinery T Technology > TS Manufactures
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Manufacturing and Materials Engineering
Depositing User:	Prof. Ir. Dr. Mohammad Yeakub Ali
Date Deposited:	27 Mar 2013 10:52
Last Modified:	27 Mar 2013 10:52
URI:	http://irep.iium.edu.my/id/eprint/28907

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