BPE-P01: Optimization of media for mass production of Schizophyllum commune using response surface methodology (RSM)

Mohd Kamal, Irnada Syuhaidah and Abdul Halim, Khairul Hazwat and Mohd Nor Rashid, Noraswati and Mel, Maizirwan (2012) BPE-P01: Optimization of media for mass production of Schizophyllum commune using response surface methodology (RSM). In: Malaysian International Conference on Trends in Bioprocess Engineering (MICOTriBE) 2012, 3-5 July 2012, Langkawi.

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Abstract

Schizophyllum commune (SC) is an edible mushroom which can be found worldwide except Antactica area. This type of mushroom has attracted attention because of its medicinal important. Optimization of the media composition is required for the higher production of SC mycelium mass in order to meet the demand particularly in pharmaceutical industries. Glucose and yeast extract was selected as carbon and nitrogen sources for the growth of this fungus and were optimised firstly using OFAT (one factor at a time) method. This media composition were then optimized using a response surface methods (RSM) to find the best composition of factors such as inoculum (seed), carbon, nitrogen concentration to produce a maximum mycelium mass as a response. Thirty set of different range medium composition were conducted by RSM design using Design Expert software. The statistical analysis showed that optimum media containing 11.7 % (v/v) of inoculum, 27g glucose and 1.2g yeast extract has given maximum production of mycelium mass where the yields of mycelium mass increased from 6.148g/l (unoptimized) to 15.68g/l in medium (optimized)

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	4085/25818
Uncontrolled Keywords:	Design Expert, Glucose, Mycelium, OFAT, RSM, Yeast extract
Subjects:	S Agriculture > S Agriculture (General) T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Maizirwan Mel
Date Deposited:	07 Dec 2012 14:27
Last Modified:	07 Dec 2012 14:27
URI:	http://irep.iium.edu.my/id/eprint/25818

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