Abdul Karim, Mohamed Ismail and Jasni, Farah Izora and Jamal, Parveen
(2011)
Fermentation of vinegar from star fruit (averrhoa carambola).
In:
Current research and development in biotechnology engineering at IIUM.
IIUM Press, Kuala Lumpur, pp. 207-210.
ISBN 9789674181444
Abstract
Fermentation of star fruit juice was conducted to produce vinegar using the yeast
Saccharomyces cerevisiae and Acetobacter aceti bacteria. The acetic fermentation was carried
out for 120 hours in shake flasks with ethanol concentration of 4, 6 and 8% (v/v), inoculums
size of 6, 8 and 10% or ml (v/v) and agitation of 150, 200 and 250 rpm. Two level factorial
designs from Design Expeli are applied for experimental design and analyses of acetic acid
and ethanol were conducted using ANOVA analysis to select the optimized results. The
experiment showed that the optimum conditions for acetic fermentation is with 8% (v/v) of
ethanol and 10% inoculums (v/v) and 250 rpm agitation speed. The vinegar obtained had a
concentration of acetic acid of 2.855% which was higher than predicted value obtained from
the selected fennented star fruit juice and the efficiency of acetic fermentation was 32.5%.
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