Mirghani, Mohamed Elwathig Saeed and Zulkifli, Mohamad Zul Fahmi
(2011)
Investigations on spider house for anti microbial activity.
In:
Current research and development in biotechnology engineering at IIUM.
IIUM Press, Kuala Lumpur, pp. 117-128.
ISBN 9789674181444
Abstract
The purpose of this new investigation was to determine if spider webs exhibit
antibacterial properties. In order to determine antibacterial properties, the spider webs
were extracted with different solvents such as methanol, ethanol, acetone, and water in
different conditions (extraction time, and concentration used for optimization). These
extracts were screened for antibacterial activity using disc diffusion assay. Two bacteria
were used in the antibacterial assay namely Bacillus subrilis, and Escherichia coli The
determination of spider webs exhibiting antibacterial properties was based, at least in this
study, solely upon the definite appearance of an inhibition zone around the well of plates.
In screening, acetone solvent was shown the best antibacterial activity compare to other
solvents with 10 mm of diameter of inhibition zone for Bacillus subtilis and 9 mm of
diameter of inhibition zone for Escherichia coli. In optimization, the maximum inhibition
zone on the Bacillus subtilis was 15 rom at a time of 48 hours and concentration of 0.035
g/ml. Meanwhile, the maximum diameter of inhibition zone on the Escherichia coli was
12 mm at a time of 48 hours and concentration of 0.035 g/ml. Therefore, this study
showed that spider webs could be potential source of new antibacterial agents.
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