Antioxidant activity of leaves of Calophyllum Rubiginosum

Bakhtiar, M. Taher and Darnis, Deny Susanti and Arief Ichwan, Solachuddin Jauhari and Ahmad, Farediah (2010) Antioxidant activity of leaves of Calophyllum Rubiginosum. American Journal of Applied Sciences , 7 (10). pp. 1305-1309. ISSN 1554-3641(O), 1546-9239 (P)

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Abstract

Calophyllum species have been used in traditional medicine for their therapeutic values for many years. This use was explained by several previous studies where antimicrobial, antifungal, anti-HIV and anti-cancer and antioxidant compounds were isolated from several Calophyllum species. Approach: The leaves were extracted with n-hexane (hexane), dichloromethane (DCM) and methanol using soxhlet apparatus. Total phenolic contents of each extract were evaluated using Folin Ciocalteau Reagent (FRC) while Ferric Thiocyanate (FTC) and 2,2-Diphenyl-1 Picrylhydrazyl (DPPH) were used for lipid peroxidation and free radical scavenging activity respectively. Results: As the results, methanol extracts (5 mg mL-1) has higher phenolic contents of 66.2 μg mg-1 Gallic Acid Equivalent (GAE) compared to n-hexane and DCM extracts with phenolic content of 50.5 and 52.5 μg mg-1 GAE respectively. In FTC method, methanol had the lower absorbance values which indicate high antioxidant activities. In DPPH radical scavenging test however, DCM extract showed high antioxidant activity with an IC50 value of 0.11mg mL-1, followed by methanol and hexane with IC50 values of 0.23 and 4.5 mg mL-1 respectively. The IC50 of standards were 0.74 and 0.56 mg mL-1 for ascorbic acid and α tocopherol, respectively. Conclusion: Finally, the positive outcome of the research can be further tested in the future to ascertain the active compound of the leaves of C. rubiginosum.

Item Type:	Article (Journal)
Additional Information:	5209/1981
Uncontrolled Keywords:	Antioxidant activity, Calophyllum rubiginosum, folin ciocalteau reagent(FRC), Ferric thiocyanate(FTC), Total Phenolic Compound(TPC), phytochemical screening.
Subjects:	R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Technology Kulliyyah of Science Kulliyyah of Science > Department of Biomedical Science
Depositing User:	Dr. Solachuddin J.A. Ichwan
Date Deposited:	04 Oct 2011 10:21
Last Modified:	06 May 2012 14:42
URI:	http://irep.iium.edu.my/id/eprint/1981

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