Regeneration of Jatropha curcas using leaf explants

Pappusamy, Arokiaraj and S.A, Lydia and L., Norshahidah and K, Latiffah (2010) Regeneration of Jatropha curcas using leaf explants. In: IIUM Research, Innovation & Invention Exhibition (IRIIE 2010), 26 - 27 January 2010, Kuala Lumpur.

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Abstract

A tissue culture protocol for regeneration of Jatropha curcas using leaf explants via somatic embryogenesis was established. The best response for callus induction from leaf explants was on Murashige and Skoog (MS) medium containing BA (1.0 and 2.0 mg l-1) and IBA (1.0 mg l-1). Callus was maintained in light and dark on MS medium supplemented with 0.5 mg l-1 BA and 0.5 mg l-1 IBA. The best response for shoot induction and shoot multiplication was on treatments containing (1.0 – 2.0 mg l-1) BA. The shoots were elongated and rooted on ½ strength MS medium containing 1.0 mg l-1 NAA (Napthalene Acetic Acid).

Item Type:	Conference or Workshop Item (Other)
Additional Information:	5357/15906
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Ms Rosyidah Taju Rahim
Date Deposited:	30 Jan 2012 09:46
Last Modified:	30 Jan 2012 09:46
URI:	http://irep.iium.edu.my/id/eprint/15906

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