Fabrication of ceramic membrane chromatography for biologics purification

Mel, Maizirwan and Abdeen, Fouad R. H. and Sopyan, Iis (2011) Fabrication of ceramic membrane chromatography for biologics purification. IIUM Engineering Journal, 12 (4). pp. 115-124. ISSN 1511-788X

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Abstract

Chromatography is one of the most important separation processes of choice for the recovery/purification of proteins and complex bio-structures. Fabrication of chromatographic membranes and their efficiency in the chromatography process has been the subject of many recent researches. In this study, a coin-like, 13 mm diameter and 3 mm thick, ceramic membrane was fabricated to be used as a chromatographic medium. The membrane is used to replace the conventional resin-based chromatography columns. Hydroxyapatite (HA) powder was used as a material for the membrane fabrication. In this project, a HA powder was produced using starch as pore creating agents. Characterization processes were done for the ceramic membrane using the suitable apparatuses. Three parameters of the fabrication process (starch wt %, compaction pressure and sintering temperature) were manipulated to optimize the performance of the membrane. The fabricated membrane was placed in a (FPLC) system to be tested for its performance as an adsorptive membrane. (IMAC) process was run by immobilizing Ni2+ ions at the membrane particles surfaces. NP protein of the (NDV) was used to test the membrane’s ability to bind Histidine-tagged proteins. The optimum set of process parameters that yielded in the highest porosity and good chromatogram was determined to be 5 wt % starch, 3000 psi compaction pressure and 1100°C sintering temperature.

Item Type:	Article (Journal)
Additional Information:	4085/13933
Uncontrolled Keywords:	membrane chromatography; porous ceramic membrane; IMAC; hydroxyapatite
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Maizirwan Mel
Date Deposited:	04 Jan 2012 14:42
Last Modified:	04 Jan 2012 14:42
URI:	http://irep.iium.edu.my/id/eprint/13933

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