Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor

Mel, Maizirwan and Arifin, Mohd Azmir and Kamaruddin, Mohd Hider and Abdul Karim, Mohamed Ismail and Yusof, Faridah (2011) Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor. African Journal of Biotechnology, 10 (81). pp. 18824-18828. ISSN 1684–5315

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Abstract

The production of monoclonal antibody (MAbs) for anti-cancer by fermentation of hybridoma cell in bench-top bioreactor was investigated. The main aim of this study was to increase the percentage of cell viability and to achieve high yield of IgM. Many ways can be applied to improve the process and one of them is by choosing the correct six sigma’s design of experiment (DOE). In this study, Taguchi’s experimental design was applied to achieve high percentage of cell viability in the fermentation experiment. The process capability of this study was later analyzed by using the MiniTab Software (Ver. 14). Analysis showed that this study fulfilled a minimum target of process improvement as indicated by the Cp and Cpk value of 0.90 and 0.86. This proved that the fermentation experiment has achieved cell viability of more than 80% throughout the study.

Item Type:	Article (Journal)
Additional Information:	4085/13646
Uncontrolled Keywords:	Monoclonal antibody, hybridoma, process capability, Taguchi experimental design.
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Maizirwan Mel
Date Deposited:	28 Dec 2011 23:06
Last Modified:	06 Jul 2012 10:43
URI:	http://irep.iium.edu.my/id/eprint/13646

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