Ahmad Fadzillah, Nurrulhidayah and Windarsih, A. and Warmiko, H.D. and Indrianingsih, A.W. and Rahmawat, L. and Volkandari, S.D. and Anggraeni, A.S. and Suratno, Suratno and Rohman, A. and Sebayang, R.A. Br (2026) Non-targeted lipidomics using LC-orbitrap HRMS and chemometrics to discriminate beef, pork, and goat meat for halal authentication = Lipidómica no dirigida mediante LC-Orbitrap HRMS y quimiometría para discriminar carne de vacuno, cerdo y cabra para la autenticación halal. Grasas Y Aceite, 77 (1-2). pp. 1-20. ISSN 0017-3495 E-ISSN 1988-4214
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Abstract
This study presents a non-targeted lipidomic approach using liquid chromatography-high-resolution mass spectrometry (LC-HRMS) coupled with chemometric analysis to discriminate between beef, pork, and goat meat. Lipid extracts from the three meat types were analyzed using reversed-phase LC-HRMS, generating comprehensive lipid profiles. Chemometrics methods, including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA), were employed to identify distinct lipid signatures capable of differentiating the meat samples. The results revealed various lipid classes, and triglycerides became the highest percentage of lipids contained in pork obtained from the positive ionization mode. Compounds of TG (52:1COOH_18:0), PE (O_36:1) and, DG (17:0_18:2) showed good capacity for discriminating pork, and further potential as biomarker candidates. The resulting models demonstrated high classification accuracy, highlighting specific lipid species which contribute significantly to the discrimination. These findings directly support the potential application for halal authentication in the meat industry, as the potential lipid markers can be further validated in binary mixtures, processed products, and real-market samples.
| Item Type: | Article (Abstract) |
|---|---|
| Uncontrolled Keywords: | Chemometrics; LC-HRMS; Lipid biomarker; Non-targeted; lipidomics; Pork. |
| Subjects: | BPH Islamic Economics > BPH121 Animal products. Halal issues Q Science > QD Chemistry |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | International Institute for Halal Research and Training (INHART) |
| Depositing User: | Dr Nurrulhidayah Ahmad Fadzillah |
| Date Deposited: | 08 Oct 2026 10:05 |
| Last Update: | 06 Nov 2026 09:59 |
| Queue Number: | 2026-10-Q6029 |
| URI: | http://irep.iium.edu.my/id/eprint/131753 |
| Indexed In: | WOS and SCOPUS, Google Scholar |
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