The effect of disinfection protocols on dimensional accuracy of irreversible hydrocolloids and Candida albicans colonisation

Norfaezah, Ahmad and Ahmad Zawawi, Nur Dini and Muhammad, Nur Farah Athirah and Nasir, Siti Hajjar and Arzmi, Mohd Hafiz (2024) The effect of disinfection protocols on dimensional accuracy of irreversible hydrocolloids and Candida albicans colonisation. IIUM Journal of Orofacial and Health Sciences, 5 (2). pp. 142-152. E-ISSN 2735-0584

PDF (Article) - Published Version Restricted to Registered users only Download (333kB) | Request a copy

Abstract

Irreversible hydrocolloid is a widely used impression material in dental practices. However, improper disinfectant protocols may affect the dimensional accuracy of the cast and increase susceptibility towards cross-infection. This study investigated the effect of disinfectants and disinfection protocols on the dimensional accuracy of alginate impression and Candida albicans biofilm formation. For methodology, an alginate impression was developed using an acrylic maxillary edentulous master cast with 3 reference points. 60 3cm round alginate beads were synthesised and treated with 2% Aseptoprint, 1% sodium hypochlorite (NaOCl) or 3% MD 520 solutions for 2 min, 1 h, 6 h, and 24 h. For the antibiofilm assay, beads were inoculated with C. albicans (ATCC MYA 4901) for 24 h, and Colony-Forming Units (CFUs) were counted using a haemocytometer, then analysed via two-way Analysis of Variance (ANOVA). Dimensional accuracy was assessed by treating the developed alginate impressions in 2% Aseptoprint, 1% NaOCl, and 3% MD 520, respectively, for 2 min, 1 h, 6 h, and 24 h. Three linear measurements were obtained and compared against the master cast and analysed statistically using Friedman Tests. The results showed that a significant reduction of CFUs was recorded after disinfection with 3% MD 520 and 2% Aseptoprint (P < 0.05). Dimension of alginate impressions was significantly changed after 6 and 24 h of disinfection time. In conclusion, the use of 3% MD 520 and 2% Aseptoprint with 1 hour immersion time is recommended for effective alginate impression disinfection with minimal change in dimensional accuracy.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Candida albicans, dimensional accuracy, infection control, irreversible hydrocolloid
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences Kulliyyah of Dentistry > Department of Orthodontics Kulliyyah of Dentistry > Department of Prosthodontics
Depositing User:	Dr Norfaezah Ahmad
Date Deposited:	09 Oct 2024 14:21
Last Modified:	10 Oct 2024 10:00
URI:	http://irep.iium.edu.my/id/eprint/114935

Actions (login required)

View Item