Minimum bactericidal and fungicidal activity of theobromine on Candida albicans and Streptococcus mutans

Gazaini, Ashraf Adily and Mohd Ali, Azrul Safuan and Syed Bakhtiar Ariffin, Syed Bazli Alwi and Arzmi, Mohd Hafiz (2024) Minimum bactericidal and fungicidal activity of theobromine on Candida albicans and Streptococcus mutans. International Journal of Allied Health Sciences (IJAHS), 8 (3). p. 97. E-ISSN 2600-8491

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Abstract

PURPOSE:Theobromine is an active compound isolated from Theobroma cacao(cacao).Even though it is known to be safe, however, the efficacy in orthodontic applications has remained unknown. Thus, this study aims to determine the effect of theobromine on C. albicans and. S. mutans that are majorly isolated from white spot lesion.METHOD:C. albicans (ATCC MYA-4901) and S. mutans (Ingbritt) were grown in yeast peptone dextrose (YPD) and brain heart infusion (BHI) broth, respectively, at 37ºC for 24 h.The microorganism was then standardized at OD620nm 0.1, equivalent to 106cells/mL and 107cells/mL of C. albicansand S. mutans,respectively.A total of 60 μL of inoculum, 60 μL of 1 g/mL of theobromine, and 60 μL of broth were pipetted into the well, and 60 μL microorganism was inoculated to 96-well plate.The theobromine was subjected to serial dilution to give a total concentration of 50% (v/v), 25% (v/v), 12.5% (v/v) and 6.25% (v/v).Finally, the plate was incubated at 37ºC for 24 h, and the OD was measured using a spectrophotometer.RESULTS:The minimum inhibitory concentration of C. albicans and S. mutans towards theobromine was observed at 500 mg/mL for both microorganisms.Furthermore, the microorganisms were also found to be susceptible to CHX.CONCLUSION:In conclusion, theobromine inhibited C. albicans and S. mutans, thus potentially be used for orthodontic application.

Item Type:	Article (Abstract)
Uncontrolled Keywords:	Theobromine, minimum inhibitory concentration, orthodontics, Candida albicans, Streptococcus mutans
Subjects:	Q Science > QR Microbiology > QR180 Immunology R Medicine > RK Dentistry R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences Kulliyyah of Dentistry > Department of Orthodontics
Depositing User:	DR AZRUL SAFUAN MOHD ALI
Date Deposited:	06 Sep 2024 11:21
Last Modified:	06 Sep 2024 11:27
URI:	http://irep.iium.edu.my/id/eprint/114295

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