A comparative study of protein-rich extract using food grade extraction procedure from marine algae, Ulva lactuca (Chlorophyta): screening through a two-level factorial experimental design strategy

Shukor, Muhammad Idham and Darnis, Deny Susanti and Mohammad Noor, Normawaty and Aminudin, Nurul Iman and Bakhtiar, M. Taher (2024) A comparative study of protein-rich extract using food grade extraction procedure from marine algae, Ulva lactuca (Chlorophyta): screening through a two-level factorial experimental design strategy. In: 4th International Conference on Chemical Processing and Engineering, IC3PE 2022, 27 September 2022, Hybrid, Yogyakarta.

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Abstract

Seaweed or macroalgae are rich in valuable bioactive compounds like proteins, carotenoids, and phenolics, making them of high commercial interest. This study focuses on investigating the extraction of protein components from the green macroalgae species Ulva lactuca using a conventional extraction method with food-grade procedures. We examined the impact of three operational variables: temperature (30–70 °C), duration (1–3 hours), and solute-to-solvent ratio (1-10% w/v) on the extract yield, determined gravimetrically through Kjeldahl analysis. The analysis revealed that the optimal extraction conditions were at 70 °C for 3 hours with a 10:100 (g/mL) solute-to-solvent ratio. The key factors in the extraction process were identified using a Two-Level Factorial design, with the solute-to-solvent ratio showing significant effects and the optimum condition at 10:100 (g/mL). Extraction duration and temperature, however, had a less significant impact on protein yields. The experimental and predicted results were in good agreement, confirming the reliability of the corresponding regression models for prediction. This protein fraction can be further concentrated and purified for use in food formulations.

Item Type:	Proceeding Paper (Slide Presentation)
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Technology Kulliyyah of Science Kulliyyah of Science > Department of Chemistry
Depositing User:	Dr Deny Susanti Darnis
Date Deposited:	06 Aug 2024 16:00
Last Modified:	06 Aug 2024 16:44
URI:	http://irep.iium.edu.my/id/eprint/113721

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