Irnawati, Irnawati and Windarsih, Anjar and putri, Angita Rosiana and Ahmad Fadzillah, Nurrulhidayah and Azmi, Aida Azrina and Kusbandari, Aprilia and Rohman, Abdul
(2024)
The use of spectroscopic methods in combination with multivariate data analysis for determination of omega fatty acids: a review.
Journal of Applied Pharmaceutical Science, 14 (1).
pp. 45-53.
ISSN 2231-3354
Abstract
Omega-3 fatty acids (ω-3 FAs), typically found in fish oils and marine-based products, are important fatty acids due
to their beneficial activities toward human health, such as anti- inflammation, immune-stimulant, lowering the risk of
cardiovascular disease and reducing blood pressure. Therefore, the determination of ω-3 FAs for quality control of
products containing these FAs is very important. Molecular spectroscopic methods offered simple, fast, and reliable
analytical methods for quality controls of food and pharmaceutical products containing ω-3 FAs since a large amount
of information could be retrieved from molecular spectra. This review highlighted the employment of molecular
spectroscopy such as near-infrared (NIR), Fourier transform infrared (FTIR), Raman, and nuclear magnetic resonance
(NMR) spectrometer combined with multivariate data analysis or chemometrics for analysis of ω-3 FAs in fish oilbased products. From this review, it is reported that the combination of molecular spectroscopy and chemometrics
could be used as effective analytical techniques for the analysis of ω-3 FAs, especially eicosapentaenoic acid (C20:5,
ω-3) and docosahexaenoic acid (C22:6, ω-3), with high accuracy and high precision. The results of quantitative
analysis of ω-3 FAs from NIR, FTIR, Raman, and NMR were comparable to those reference results obtained from
gas chromatography-mass spectrometry measurement. In the future, collaborative studies through proficiency testing
should be performed to get standardized methods based on molecular spectroscopy and chemometrics.
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