Mohd Fuad, Aalina Sakiina and Wan Mazlan, Wan Nur Akma and Abdul Wahab, Ridhwan and Nasruddin, Nurrul Shaqinah and Arzmi, Mohd Hafiz (2023) The effect of probiotic Lactobacillus rhamnosus GG on Candida albicans and Candida tropicalis biofilm formation: a preliminary study. IIUM Journal of Orofacial and Health Sciences, 4 (2). pp. 126-131. E-ISSN 2735-0584
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Abstract
Lactobacillus rhamnosus is Gram-positive and lactic acid-producing bacterium. Meanwhile, Candida albicans and Candida tropicalis are opportunistic fungi that cause oral candidiasis. This study aimed to determine the effect of L. rhamnosus GG (LGG) on the biofilm formation of C. tropicalis and C. albicans with the hypothesis that LGG inhibits the biofilm of the yeasts. C. albicans ATCC MYA-4901 and C. tropicalis ATCC 13803 were standardised to 1x106 cells to form a mono-species biofilm. LGG was standardised to 1x107 cells, equivalent to absorbance 0.5 at OD620nm. The microorganisms were cultivated in nutrient broth in a 96-well plate and incubated at 37°C for 24 h and 48 h. Co-culture biofilm was developed by combining Candida spp. with LGG in the same well at a similar concentration as the mono-culture. Crystal violet assay was conducted to assess the biofilm biomass with absorbance measured at OD620nm wavelength. After 24 hours, polymicrobial biofilms of C. albicans with LGG decreased by 37.1 ± 9.2%. At 48 hours, it further decreased to 44.7 ± 5.9%. For C. tropicalis, co-culture biofilms with LGG decreased by 16.3 ± 5.9% and 35.7 ± 7.6% after 24 h and 48 h incubation, respectively. LGG significantly reduced C. albicans biofilm compared to C. tropicalis (P<0.05). In conclusion, LGG has antibiofilm activity against C. albicans and C. tropicalis. However, further study is needed to conclude the effect against other species strains.
| Item Type: | Article (Journal) |
|---|---|
| Additional Information: | 7855/109436 |
| Uncontrolled Keywords: | biofilm, Candida albicans, Candida tropicalis, Lactobacillus rhamnosus GG, probiotic |
| Subjects: | Q Science > QR Microbiology R Medicine > RK Dentistry |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences |
| Depositing User: | AP Ts Dr Mohd Hafiz Arzmi |
| Date Deposited: | 28 Dec 2023 15:57 |
| Last Modified: | 29 Feb 2024 15:53 |
| URI: | http://irep.iium.edu.my/id/eprint/109436 |
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