Clinical performance of reverse transcription loop mediated isothermal amplification COVID-19 assay on gold- nanoparticle-modified screen-printed Carbon Electrode using differential pulse voltammetry

Zulhairee, Munirah and Mohd Zain, Rozainanee and Che Mohamad Nor, Adibah and Jafar, Nur Fadhilah and Md Noh, Mohd Fairulnizal and Ahmad Noorden, Mohd Shihabuddin and Nordin, Anis Nurashikin and Ab Rahim, Rosminazuin and Gunawan, Teddy Surya and Chin, Lim Ying and Mohd, Yusairie and Azman, Nur Asyiqin and Mohd Zain, Zainiharyati (2023) Clinical performance of reverse transcription loop mediated isothermal amplification COVID-19 assay on gold- nanoparticle-modified screen-printed Carbon Electrode using differential pulse voltammetry. Sensors and Materials, 35 (10). pp. 4731-4750. ISSN 0914-4935 E-ISSN 2435-0869

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Abstract

The World Health Organization (WHO) has recommended real-time reverse transcription polymerase chain reaction (RT-PCR) as the gold standard for coronavirus disease detection. In this study, we aim to validate the clinical performance of reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay on gold-nanoparticle-modified screen-printed carbon electrode (AuNP/SPCE) using differential pulse voltammetry (DPV) and to compare it with real-time RT-PCR. The shape of the electrodeposited AuNP on SPCE was quasi-spherical with a size of ±500 nm. The developed RT-LAMP primer was designed from the GenBank database using the NCBI Multiple Alignment tools and Jalview software. Nasopharyngeal clinical samples were obtained from suspected COVID-19 patients (n = 148). The RT-LAMP products were dropped on the modified AuNP/SPCE under DPV setting, which resulted in current change (∆I) responses. The positive and negative samples produced significantly different ∆I signals with a p-value <0.0001 at a 95% confidence interval using Student’s t-test. The RT-LAMP assay using Au/SPCE exhibited a 30 s response time per analysis. The clinical sensitivity and specificity obtained were 79.7% and 85.1%, respectively, with a detection limit of 0.4 copies µl−1. Hence, this proposed method is suitable for COVID-19 RNA detection in resource-limited settings.

Item Type:	Article (Journal)
Uncontrolled Keywords:	COVID-19, RT-PCR, RT-LAMP, AuNP/SPCE, differential pulse voltammetry, electrochemical sensor
Subjects:	T Technology > TK Electrical engineering. Electronics Nuclear engineering > TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices > TK7885 Computer engineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering Kulliyyah of Engineering > Department of Electrical and Computer Engineering
Depositing User:	Prof. Dr. Teddy Surya Gunawan
Date Deposited:	15 Nov 2023 09:04
Last Modified:	02 Feb 2024 16:27
URI:	http://irep.iium.edu.my/id/eprint/108084

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