Optimisation of phenolic compounds and antioxidant capacity of Trigona honey and propolis using response surface methodology from fermented food products

Hagar, Abdullah and Ibrahim, Muhammad and Ahmed, Idris Adewale and Ramli, Norazsida and M. Jalil, Abbe Maleyki and Abd Rani, Nurlisa Fatihah (2021) Optimisation of phenolic compounds and antioxidant capacity of Trigona honey and propolis using response surface methodology from fermented food products. International Food Research Journal, 28 (6). pp. 1233-1244. ISSN 1985-4668 E-ISSN 2231-7546

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Abstract

Honey and propolis are honeybee products that are becoming increasingly common as a result of their ability to improve human health. The optimal combination of honey and propolis for total phenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity were analysed for Trigona honey and propolis aqueous extracts using response surface methodology and a central composite design. The effect of honey (X1: 15 - 16.5 g) and propolis (X2: 13.5 - 15 g) on the total phenolic content (TPC, Y1), total flavonoid content (TFC, Y2), antioxidant capacity (DPPH, Y3; ABTS, Y4), and FRAP (Y5) were tested. The experimental outcomes were adequately fitted into a second-order polynomial model regarding TPC (R2 = 0.9539, p = 0.0002), TFC (R2 = 0.9209, p = 0.0010), antioxidant activity (DPPH, R2 = 0.9529, p = 0.0002; ABTS, R2 = 0.9817, p < 0.0001), and FRAP (R2 = 0.9363, p = 0.0005). The optimal percentage compositions of honey and propolis were 15.26 g (50.43%) and 15 g (49.57%), respectively. The predicted results for TPC, TFC, DPPH (IC50), ABTS, and FRAP were 162.46 mg GAE/100 g, 2.29 mgQE/g, 14.52 mg/mL, 564.27 µMTE/g, and 3.56 mMTE/g, respectively. The experimental outcomes were close to the predicted results: 152.06 ± 0.55 mg GAE/100 g, 2.21 ± 0.05 mg QE/g, 13.85 ± 0.34 mg/mL, 555.22 ± 36.84 µMTE/g, and 3.71 ± 0.02 mMTE/g, respectively. It was observed that the optimal combination of honey and propolis provided the highest antioxidant yield and can be used as functional foods, cosmetics, and medical and pharmacological ingredients.

Item Type:	Article (Journal)
Uncontrolled Keywords:	phenolic content; flavonoid content; antioxidant; Trigona propolis; Trigona honey; reseponse surface methodology
Subjects:	Q Science > Q Science (General) R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Allied Health Sciences > Department of Nutrition Sciences
Depositing User:	Muhammad Ibrahim
Date Deposited:	18 Oct 2023 15:10
Last Modified:	18 Oct 2023 15:28
URI:	http://irep.iium.edu.my/id/eprint/107519

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