Chemical composition and antioxidant/antibacterial depictions of Zahidi date palm (Phoenix dactylifera) kernel oil

Alkhalaf, Maha I. and Churchill, Grant C. and Mirghani, Mohamed Elwathig Saeed (2023) Chemical composition and antioxidant/antibacterial depictions of Zahidi date palm (Phoenix dactylifera) kernel oil. Journal of King Saud University - Science, 35 (7). pp. 1-7. ISSN 1018-3647 E-ISSN 2213-686X

	PDF (Journal) - Published Version Restricted to Repository staff only Download (584kB) | Request a copy
Preview	PDF - Supplemental Material Download (167kB) | Preview

Abstract

Date palm “Phoenix dactylifera” variety Zahidi is a unique well-known cultivar. Mature fruits are semi-dry, medium size, cylindrically shaped and golden brown. However, there are three stages to harvesting the dates soft, hard or semi-soft. In this research, the oil processed from the seed/kernel of the date palm Zahidi is chemically analysed for its composition, antioxidant capacity and its antibacterial properties were investigated. Date seeds were ground and extracted for the oil. Wet chemical analyses were implemented on the extracted oil via various analytical techniques such as high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). Oil fractions of ethanol, methanol and acetone were tested for antibacterial activity, which was evaluated employing the Kirby–Bauer disc-diffusion method. Antibacterial action was examined for four bacterial strains; two of them representing Gram-positive such as Bacillus subtilis, and Staphylococcus aureus, and the other two for Gram-negative such as Pseudomonas aeruginosa and Escherichia coli. The antibiotics tetracycline, chloramphenicol, streptomycin, gentamycin and vancomycin were used as positive standards. The Minimum Bactericidal Concentration (MBC) and Minimum Inhibitory Concentration (MIC) of the bacterial strains were tested and evaluated. The antioxidant activity was evaluated as free-radical scavenging capacity (RSC) towards 2,2 - diphenyl -1- picrylhydrazyl (DPPH●) radicals for the methanol fraction (MF), remaining lipid fraction (LF) and non-fractioned oil, i.e., the total fraction (TF). The RSCs for the MF, LF and TF were 42.5, 9.1 and 68.3, respectively. Zahidi seed/kernel oil extract inhibited all of the test microorganisms, with MICs that ranged from 10 to 42 mg/mL and MBCs were found to be ranged between 21 and 167 mg/mL. These outcomes suggest that the extracts could be developed as a foundation of expected antimicrobial complexes to fight unwanted bacteria in foods. Overall, the results showed that the oil is suitable for food, cosmetics, personal care products and pharmaceuticals.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Antioxidant; antibacterial; date palm (Phoenix dactylifera); DPPH●; GC-MS; lipid fraction; seed oil.
Subjects:	BPC Science and Technology in Islam Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART)
Depositing User:	A Prof Elwathig Mirghani
Date Deposited:	07 Aug 2023 14:28
Last Modified:	07 Nov 2023 16:42
URI:	http://irep.iium.edu.my/id/eprint/105853

Actions (login required)

View Item