Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology

Mohd Dzakir, Nur Fatin Nadia and Abdullah, Erna Normaya and Hassanuddin, Nur Amanina and Shamsuri, Syamimi Sulfiza and Iqbal, Anwar and Mat Piah, Mohd Bijarimi and Ahmad, Mohammad Norazmi (2023) Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology. In: 3 rd IKMPB Online Symposium 2023 - Innovation in Chemistry Towards Sustainable Development).

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Abstract

Recently, the widespread use of proteases in industries, such as detergent, food, and pharmaceutical sectors, has increased the demand for proteolytic enzymes in the global market. Although the main sources of commercial proteases are animals and microorganisms, the potential of using nonconventional sources, especially plants, should not be overlooked. In this study, proteolytic enzymes were extracted from the sarcocarp of ridged gourd (Luffa acutangula), and the effect of the extraction process on protease activity was evaluated. The crude enzyme was optimized via response surface methodology (RSM) using a central composite rotatable design (CCRD). Four independent variables were studied, namely, the pH, the concentrations of Triton X-100 (TX-100) and 2-mercaptoethanol, and the mixing time. The optimum level of each variable based on the RSM model was determined: at pH 6.38, 4.99% (v/v) TX-100, 0.15 M 2-mercaptoethanol, and a mixing time of 4.09 min, the optimum protease activity was estimated at 1.35 U/g. A verification test revealed satisfactory agreement between the model and experimental results with 96% desirability, indicating that the quadratic model generated from RSM is significant.

Item Type:	Proceeding Paper (Invited Papers)
Uncontrolled Keywords:	Optimization; Response Surface Methodology; Central Composite Rotatable Design; Luffa acutangular; protease
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science Kulliyyah of Science > Department of Chemistry
Depositing User:	Dr Mohammad Norazmi Ahmad
Date Deposited:	11 Jul 2023 14:22
Last Modified:	03 Jan 2024 14:05
URI:	http://irep.iium.edu.my/id/eprint/105427

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