Abdul Samat, Asmak and Abdul Hamid, Zuratul Ain and Jaafar, Mariatti and Ong, Chern Chung and Yahaya, Badrul Hisham (2023) Investigation of the in vitro and in vivo biocompatibility of a three-dimensional printed Thermoplastic Polyurethane/Polylactic Acid blend for the development of Tracheal Scaffolds. Bioengineering 2023, 10 (394). E-ISSN 2306-5354
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Abstract
Tissue-engineered polymeric implants are preferable because they do not cause a significant inflammatory reaction in the surrounding tissue. Three-dimensional (3D) technology can be used to fabricate a customised scaffold, which is critical for implantation. This study aimed to investigate the biocompatibility of a mixture of thermoplastic polyurethane (TPU) and polylactic acid (PLA) and the effects of their extract in cell cultures and in animal models as potential tracheal replacement materials. The morphology of the 3D-printed scaffolds was investigated using scanning electron microscopy (SEM), while the degradability, pH, and effects of the 3D-printed TPU/PLA scaffolds and their extracts were investigated in cell culture studies. In addition, subcutaneous implantation of 3D-printed scaffold was performed to evaluate the biocompatibility of the scaffold in a rat model at different time points. A histopathological examination was performed to investigate the local inflammatory response and angiogenesis. The in vitro results showed that the composite and its extract were not toxic. Similarly, the pH of the extracts did not inhibit cell proliferation and migration. The analysis of biocompatibility of the scaffolds from the in vivo results suggests that porous TPU/PLA scaffolds may facilitate cell adhesion, migration, and proliferation and promote angiogenesis in host cells. The current results suggest that with 3D printing technology, TPU and PLA could be used as materials to construct scaffolds with suitable properties and provide a solution to the challenges of tracheal transplantation.
| Item Type: | Article (Journal) |
|---|---|
| Uncontrolled Keywords: | biocompatibility, thermoplastic polyurethane, polylactic acid, degradation, pH, Inflammatory response |
| Subjects: | Q Science > QP Physiology R Medicine > R Medicine (General) T Technology > T Technology (General) T Technology > T Technology (General) > T201 Patents. Trademarks |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences |
| Depositing User: | Dr Asmak Abdul Samat |
| Date Deposited: | 17 May 2023 21:29 |
| Last Modified: | 17 May 2023 21:29 |
| URI: | http://irep.iium.edu.my/id/eprint/104637 |
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