Response of auxins and cytokinins on Citrus suhuiensis adventitious shoot culture initiation and growth

Mohamad Puad, Noor Illi and Wan Zulkifli, Wan Majidah and M. Fathil, Nur Alia and Amid, Azura (2023) Response of auxins and cytokinins on Citrus suhuiensis adventitious shoot culture initiation and growth. Journal of Agrobiotechnology, 14 (1). pp. 21-31. E-ISSN 2180-1983

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Abstract

Bacterial and viral diseases are the common problems hampering the vast majority of citrus plants which resulted in a decrease of citrus trees development and production yield. While the usage of chemicals to eliminate internal pathogens is harmful to the ecosystem, plant tissue culture is another alternative to develop disease-free plants based on the defined physical and chemical conditions under an aseptic environment. This study aimed to initiate Citrus suhuiensis (C. suhuiensis) adventitious shoot culture specifically in response towards different types of plant growth regulators (PGRs). The effects of explants and PGRs were evaluated on the shoot growth within 35 days of culture. C. suhuiensis shoot cultures were induced from three different explants which are leaf, callus and seeds on the solid Murashige and Skoog (MS) medium containing different combinations of PGRs which are auxins i.e. 1-naphthylacetic acid (NAA) or indole butyric acid (IBA) at 0.5 mg/L, respectively with cytokinins i.e. 6-benzylaminopurine (BAP) or kinetin (KN) at various concentrations (0.5 - 4.0 mg/L). Based on the results, the earliest shoot emergence from the cotyledon can be observed after the 8th day of inoculation for PGRs combination of 0.5 mg/L IBA with 2.0 mg/L, 3.0 mg/L KN and 3.0 mg/L BAP, respectively. Meanwhile, based on the ANOVA analysis (p-value < 0.05), the most significant PGRs combination for the establishment of C. suhuiensis shoot culture is IBA and KN followed by the treatment of NAA and KN. The findings of this study can serve as a basis for future investigation of micropropagation of shoot culture and cultivation of C. suhuiensis plant.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Adventitious shoot, Citrus suhuiensis, auxins, cytokinins, plant micropropagation
Subjects:	S Agriculture > SB Plant culture T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Noor Illi Mohamad Puad
Date Deposited:	14 Apr 2023 12:02
Last Modified:	23 Dec 2023 12:20
URI:	http://irep.iium.edu.my/id/eprint/104409

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