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Statistical screening of physico-chemical factors for biohydrogen production by Enterobacter aerogenes from sago wastewater

Ulhiza, Tami Asti and Mohamad Puad, Noor Illi and Azmi, Azlin Suhaida (2022) Statistical screening of physico-chemical factors for biohydrogen production by Enterobacter aerogenes from sago wastewater. Journal of Engineering Science and Technology (JESTEC), 17 (5). pp. 3012-3024. ISSN 1823-4690 (In Press)

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Abstract

High organic content of sago wastewater (SWW) can be utilized as a substrate for biohydrogen production. After acid pre-treatment of SWW using 1.5% sulfuric acid at 121ºC for 60 minutes, its glucose content increased from 0.146 g/L to 9.36 g/L. In this study, Enterobacter aerogenes (E. aerogenes) was used as the biohydrogen producer. Screening of ten physico-chemical factors that influenced biohydrogen production were performed using Plackett-Burman design tool (Design Expert 9.0). Yeast extract concentration, fermentation temperature, and inoculum concentration were ranked as the three most significant factors. The maximum hydrogen concentration, volume production, and hydrogen yield achieved in this study were 454.30 μmol/L, 88 mL, and 2.42 mmol H2/mol glucose, respectively. The findings from this study can be used for further optimization of process conditions for biohydrogen production by E. aerogenes using SWW as the substrate.

Item Type: Article (Journal)
Subjects: T Technology > T Technology (General)
T Technology > TP Chemical technology > TP155 Chemical engineering
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Noor Illi Mohamad Puad
Date Deposited: 28 Sep 2022 08:18
Last Modified: 28 Sep 2022 08:18
URI: http://irep.iium.edu.my/id/eprint/100241

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