A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana

Amid, Azura and Warren, Garreth J. (2009) A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana. International Journal of Botany, 5 (2). pp. 144-152. ISSN 1811-9719 (O), 1811-9700 (P)

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Abstract

The sfr3-1 mutation causes freezing-sensitivity in Arabidopsis thaliana. Through mapping, sequencing and transgenic complementation, sfr3-1 was revealed as a missense mutation in ACC1, which is an essential gene encoding multifunctional acetyl-CoA carboxylase. Suppression of ACC1 expression by RNA interference produced a freezing-sensitive phenotype with some similarity to that of sfr3-1. The dCAPS primers and PCR confirmed that sfr3 gene encodes multifunctional acetyl-CoA carboxylase. Microarray and real-time PCR experiments demonstrated that the expression of ACC1 increase only 1.48 fold in wild-type and 1.35 fold in mutant in response to cold treatment. Studies also suggested that the sfr3-1 mutation is more likely to be a temperature-sensitive mutation as the sfr3 mutant cuticle becomes leaky only at low temperature and this was confirmed by cuticular defects analysis.

Item Type:	Article (Journal)
Additional Information:	3688/864
Uncontrolled Keywords:	sfr3-1 mutant, acetyl-CoA carboxylase, RNA interference. dCAPS, freezing sensitive mutant
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof Ts Dr Azura Amid
Date Deposited:	05 Aug 2011 15:20
Last Modified:	05 Aug 2011 15:21
URI:	http://irep.iium.edu.my/id/eprint/864

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