Yusof, Faridah and Abd. Aziz, Nur Amalin (2020) Enhanced Kinetic Performance of CLEA-Lipase extracted from skim latex of Hevea brasiliensis upon immobilization on magnetic nanoparticles. Journal of Advanced Research in Materials Science, 73 (1). pp. 1-11. ISSN 2289-7992
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Abstract
In this research, lipase recovered from the skim latex of Hevea brasiliensis was immobilized via cross-linked enzyme aggregates (CLEA) technology, while supported by magnetic nanoparticles {MNPs), for properties enhancement. Hybrid immobilization may have affected the kinetic performances of the biocatalysts. The kinetic performance of both MNP supported and unsupported CLEAlipase, were evaluated based on the Michaelis-Menten model using p-nitrophenyl palmitate as the substrate. Three different linearization model equations were used to compute the kinetic properties, Vmax and Km, and a hyperbolic regression was conducted with computer software. Based on the best fitted model, Vmax of MNP-CLEA-lipase, obtained from the Lineweaver-Burk plot (R2=0.9823), was 0.0023 µmol/min.mL, which is higher than CLEA-lipase (0.0015 µmol/min.mL), indicating it needs much higher substrate concentration to saturate the enzymatic sites to reach its maximum velocity. Km for MNP-CLEA-lipase was 0.4400 µmol, compared to 0.5188 µmol for CLEA-lipase, inferring that it has a higher affinity towards substrates, whereby its rate will approach V with lower substrate concentration. Overall, this research demonstrated that wasteful by-products such as skim latex can be converted to useful value-added biocatalyst. A better understanding of the kinetic parameters of this newly produced MNP immobilized biocatalyst is necessary for its further development.
| Item Type: | Article (Journal) |
|---|---|
| Additional Information: | 4734/84140 |
| Uncontrolled Keywords: | Skim latex, serum, Hevea brasiliensis, CLEA-lipase, magnetic nanoparticles (MNP), Michaelis-Menten enzyme kinetics, v_max and K_m |
| Subjects: | T Technology > TP Chemical technology T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Engineering > Department of Biotechnology Engineering Kulliyyah of Engineering |
| Depositing User: | DR FARIDAH YUSOF |
| Date Deposited: | 03 Nov 2020 15:05 |
| Last Modified: | 03 Nov 2020 15:05 |
| URI: | http://irep.iium.edu.my/id/eprint/84140 |
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