Fourier Transform Infrared Spectroscopy (FTIR) coupled with multivariate calibration and discriminant analysis for authentication of extra virgin olive oil from rambutan seed fat

A, Nurwahidah and ., Rumiyati and Riyanto, Sigit and Ahmad Fadzillah, Nurrulhidayah and Kartika, Betania and Abdul, Rohman (2019) Fourier Transform Infrared Spectroscopy (FTIR) coupled with multivariate calibration and discriminant analysis for authentication of extra virgin olive oil from rambutan seed fat. Food Research, 3 (6). pp. 727-733. E-ISSN 2550-2166

	PDF - Published Version Restricted to Registered users only Download (616kB) | Request a copy
Preview	PDF (SCOPUS) - Supplemental Material Download (274kB) | Preview

Abstract

The adulteration practice in pharmaceutical industries, especially in fats and oils used as a vehicle in some pharmaceutical products must be identified to assure its quality. In this study, Fourier Transform Infrared Spectroscopy (FTIR) in combination with chemometrics techniques of multivariate calibration and discriminant analysis (DA) were used for the authentication of extra virgin olive oil (EVOO) from rambutan seed fat (RSF). EVOO, RSF, and the mixture of EVOO-RSF were scanned using FTIR spectrophotometer at mid-infrared region (4000-650 cm-1). The results showed that normal FTIR spectra at wavenumbers region of 1446.8-1409.7 cm-1 and 2368.6-1769.9 cm-1 combined with principle component regression (PCR) offered the best quantitative model for prediction of RSF levels in EVOO. The coefficient of determination (R2) values obtained for the relationship between actual values of RSF and predicted values were of 0.9955 and 0.9915 in calibration and prediction models, respectively. The errors in calibration and prediction models were relatively low, accounting of 2.17% and 3.68%, respectively. The classification model between unadulterated or pure EVOO and adulterated EVOO with RSF was successfully carried out using DA at wavenumbers of 3100-1000 cm-1 without any samples mistakenly classified into the wrong group. FTIR spectroscopy in combination with chemometrics offered effective tools for authentication of EVOO against the adulteration practice.

Item Type:	Article (Journal)
Additional Information:	7619/77434
Uncontrolled Keywords:	Olive oil, Rambutan seed fat, PCR, Discriminant analysis, Authentication
Subjects:	S Agriculture > S Agriculture (General) T Technology > TS Manufactures
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART)
Depositing User:	Dr Nurrulhidayah Ahmad Fadzillah
Date Deposited:	19 Mar 2020 23:45
Last Modified:	11 Aug 2020 10:32
URI:	http://irep.iium.edu.my/id/eprint/77434

Actions (login required)

View Item