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Anti-neoplastic potential of ethyl-p-methoxycinnamate of Kaempferia galanga on oral cancer cell lines

Ichwan, Solachuddin J. A. and Husin, Atiyah and Suriyah, Wastuti Hidayati and Lestari, Widya and Omar, Muhammad Nor and Kasmuri, Abdul Razak (2019) Anti-neoplastic potential of ethyl-p-methoxycinnamate of Kaempferia galanga on oral cancer cell lines. In: Conference on BioMedical and Advanced Materials (Bio-CAM 2017), 28-29th November 2017, Pulau Langkawi, Kedah.

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Abstract

Kaempferia galanga is one of the herbal plant that have many medicinal properties. The plant extract has also been reported to possess in vitro anti-cancer activities on various cancer cell lines such as human colon cancer SW620, cervical cancer C33A, and breast cancer MCF-7 cell lines. Ethyl-p-methoxycinnamate (EPMC), a major constituent of volatile oil of Kaempferia galanga has been attributed to its medicinal benefits. We report here for the first time the anticancer potential of EPMC on oral cancer cells derived from human oral cavity, HSC-3 and Ca922 lines. MTT assay results showed that EPMC markedly induced cytotoxicity on HSC-3 (IC50 0.075 mg/mL) and Ca922 (IC50 0.085 mg/mL) cell lines. The apoptotic activity induced by EPMC was detected in both cell lines by flow cytometry and confirmed with Caspase 3/7 assay. Moreover, quantitative RT-PCR analysis revealed that EPMC-induced apoptosis in both lines elevated the mRNA levels of pro-apoptotic genes caspase-9 and PUMA. These results suggest that EPMC may be a potential candidate for oral cancer treatment.

Item Type: Conference or Workshop Item (Plenary Papers)
Additional Information: 5731/74084
Uncontrolled Keywords: Kaempferia galanga; ethyl-p-methoxycinnamate; oral cancer cell lines; cytotoxicity; apoptosis
Subjects: R Medicine > RK Dentistry
R Medicine > RM Therapeutics. Pharmacology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Dentistry
Kulliyyah of Dentistry > Fundamental Dental and Medical Sciences
Kulliyyah of Pharmacy
Kulliyyah of Science
Depositing User: Dr. Solachuddin J.A. Ichwan
Date Deposited: 19 Sep 2019 09:54
Last Modified: 19 Sep 2019 09:54
URI: http://irep.iium.edu.my/id/eprint/74084

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