In vitro evaluation of the effect of Pluchea indica extracts in promoting glucose consumption activity on a liver cell line

Suriyah, Wastuti Hidayati and Ichwan, Solachuddin J. A. and Kasmuri, Abdul Razak and Taher, Muhammad (2019) In vitro evaluation of the effect of Pluchea indica extracts in promoting glucose consumption activity on a liver cell line. Makara Journal of Health Research, 23 (1). pp. 48-52. ISSN 2356-3664 E-ISSN 2356-3656

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Abstract

Background: Type 2 diabetes mellitus is a chronic metabolic disorder that is associated with mortality and morbidity. Recently, several plant-based agents have been used in the management of diabetes. Pluchea indica has been traditionally consumed as a medicinal plant in Southeast Asia, and its leaves have demonstrated induction of hypoglycemic effect in normal rats. This in vitro study aimed to evaluate the potency of P. indica extracts in stimulating glucose consumption in human liver CCL-13 cell line model. Methods: P. indica leaves were dried and extracted using a series of organic solvents and water. The effect of the extracts on cell viability was determined by MTT assay. The glucose consumption was analyzed using glucose oxidase method. Results: Our results revealed that the methanol extract of P. indica could significantly increase glucose consumption of cells in a concentration-dependent manner, which suggests the usefulness of the extract as an antidiabetic candidate via stimulation of glucose uptake into the liver cells. Conclusion: Our study suggests that P. indica is a potential natural candidate for diabetes mellitus management.

Item Type:	Article (Journal)
Additional Information:	5731/71834
Uncontrolled Keywords:	diabetes mellitus; type 2; liver; cell line; glucose oxidase; Pluchea indica
Subjects:	R Medicine > RM Therapeutics. Pharmacology R Medicine > RM Therapeutics. Pharmacology > RM300 Drugs and their action
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences
Depositing User:	Dr. Solachuddin J.A. Ichwan
Date Deposited:	25 Apr 2019 16:28
Last Modified:	31 Jul 2019 15:53
URI:	http://irep.iium.edu.my/id/eprint/71834

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