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Anti-proliferative activity of triterpenes isolated from Clinicanthus nutans on Hep-G2 liver cancer cells

Zakaria, Khairun Najwa and Amid, Azura and Zakaria, Zubaidah and Jamal, Parveen and Ismail, Azli (2019) Anti-proliferative activity of triterpenes isolated from Clinicanthus nutans on Hep-G2 liver cancer cells. Asian Pacific Journal of Cancer Prevention, 20 (2). pp. 563-567.

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Abstract

Problem statement: Clinicanthus nutans has been used by Malaysian since long time ago. It is used to treat many diseases including cancer. Many studies carried out on its crude extract but no clear report on the specific secondary metabolites responsible for its nature in treating selected diseases. Objective: This study aims to confirm the practice carried out by many people on the usage of Clinicanthus nutans in treating cancer. Methods: C. nutans leaves were extracted by methanol. Thin layer chromatography was used to identify the suitable solvent for fractions separation. The fractions were then separated at larger volume using gravity column chromatography. Each fraction was tested on its anti-proliferative activity on Hep-G2 liver cancer cells by MTT assay. The phytochemical screening was carried out to identify the bioactive compound based on qualitative analysis. Results: The fraction 2 (F2) of C. nutans showed the lowest IC50 value of 1.73 µg/ml against Hep-G2 cancer cells, and it is identified as triterpenes. Conclusion: The fraction F2 identified as triterpenes isolated from C. nutans has potential as an anti-proliferative agent against liver cancer.

Item Type: Article (Journal)
Additional Information: 3688/70977
Uncontrolled Keywords: Clinicanthus nutans- anti-proliferative- triterpenes- liver cancer
Subjects: T Technology > TP Chemical technology
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes: International Institute for Halal Research and Training (INHART)
Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Azura Amid
Date Deposited: 08 Mar 2019 16:23
Last Modified: 12 Jul 2019 15:49
URI: http://irep.iium.edu.my/id/eprint/70977

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