High yield of second-generation ethanol in an ionic liquid-cellulase integrated system for single-step processing of empty fruit bunch

Elgharbawy, Amal A.M. and Alam, Md Zahangir and Moniruzzaman, Muhammad and Kabbashi, Nassereldeen Ahmed and Jamal, Parveen (2019) High yield of second-generation ethanol in an ionic liquid-cellulase integrated system for single-step processing of empty fruit bunch. Biofuels, 2019. pp. 1-13.

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Abstract

Ionic liquids (ILs) are regarded as green solvents and have demonstrated ability in the solvation of lignocellulosic material, thus promoting its enzymatic hydrolysis by cellulases. In this study, cellulase (PKC-Cel) obtained from Trichoderma reesei (RUTC30) through fermentation displayed good stability in choline acetate [Cho]OAc, a biodegradable IL. Empty fruit bunches (EFB) were subjected to single-step hydrolysis in an IL-enzyme system. Response surface methodology was employed to maximize ethanol production. Three parameters were optimized, namely sugar loading, inoculum concentration, and agitation. The distilled ethanol purity was 98.6% (v/v). The ethanol yield was 87.94% of the theoretical yield, which was 0.275 g ethanol g−1 EFB. In comparison, the non-IL system produced 0.127 g g−1 EFB. The yield coefficients Yps, Yxs, and Ypx were 0.552, 0.148, and 3.69 g g−1. The specific growth rate (μ) was determined to be 0.264 h−1 and the (μmax) was 0.777 h−1.

Item Type:	Article (Journal)
Additional Information:	8637/70703
Uncontrolled Keywords:	Cellulase, compatible, pretreatment, ionic liquids, hydrolysis, bioethanol
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr AMAL ELGHARBAWY
Date Deposited:	06 Mar 2019 09:04
Last Modified:	06 Mar 2019 09:04
URI:	http://irep.iium.edu.my/id/eprint/70703

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