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Procedure to develop binodal curve and phase diagram for aqueous two-phase system

Mohd Arshad, Zatul Iffah and Amid, Azura (2018) Procedure to develop binodal curve and phase diagram for aqueous two-phase system. In: Multifaceted protocol in biotechnology. Springer Nature, Singapore, pp. 75-82. ISBN 978-981-13-2256-3

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Purification of protein is normally involved an expensive downstream processing step. For large scale manufacturing facilities, identification of an economical procedure is the most advantage. Aqueous Two-Phase System (ATPS) is a technique that suitable to be used in extracting protein from the mix culture supernatant. The process is also economical. However, there are a few tricky parts that the researchers need to master before this technique can be applied. Therefore, this chapter presents the step by step procedure in optimizing this technique.

Item Type: Book Chapter
Additional Information: 3688/70269
Uncontrolled Keywords: Purification · Recombinant protein · Biodal curve · Solvent-solvent extraction
Subjects: T Technology > TP Chemical technology > TP200 Manufacture and use of chemicals
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Azura Amid
Date Deposited: 11 Feb 2019 16:36
Last Modified: 11 Feb 2019 16:36
URI: http://irep.iium.edu.my/id/eprint/70269

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