Quantifying E. coli O157:H7 via linear sweep voltammetry in phosphate buffered saline and tap water

Ali Ahmed, Nasteho and Ramli, Nurul Izzati and Abdul Jalal, Nurfatin Azma and Abd. Wahab, Mohd. Firdaus and Wan Salim, Wan Wardatul Amani (2018) Quantifying E. coli O157:H7 via linear sweep voltammetry in phosphate buffered saline and tap water. In: Proceedings of the International Conference Biotechnology Engineering, ICBioE ‘18, 19-20 September 2018, Gombak, Kuala Lumpur.

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Abstract

E. coli O157:H7 is a common microorganism that is responsible for contaminating water after major floods events. Standard methods of pathogen detection cannot be utilized in the field; the need for a portable immunosensor becomes urgent so detection of pathogens can be done at remote post-flood areas with no lab and technician access. In this study, the objectives are to analyze E. coli binding to reduced graphene oxide (rGO) electrode surfaces via Linear Sweep Voltammetry (LSV) in phosphate buffered saline (PBS), pH 7.4, and potable tap water, and to correlate voltammetry results with E. coli attachment on electrodes by using FESEM. From the results, LSV in PBS shows sensitivity of 38.7 µA/(cell/ml) in comparison to tap water, with a lower sensitivity of 2.20 µA/(cell/ml). Linear range from 103 to 108 cells/µl was achieved in both solution types. FESEM results show higher attachment of cells on rGO-based electrodes for higher concentration of cells, confirming the lowering of current in LSV measurements owing to increased cell attachment.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	5236/68448
Uncontrolled Keywords:	Escherichia coli, Linear sweep voltammetry, Immunosensor, Phosphate buffered saline, Tap water
Subjects:	Q Science > QD Chemistry T Technology > TD Environmental technology. Sanitary engineering > TD169 Environmental protection T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Wan Wardatul Amani Wan Salim
Date Deposited:	18 Dec 2018 15:17
Last Modified:	03 Jul 2019 15:29
URI:	http://irep.iium.edu.my/id/eprint/68448

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