Effect of extraction process parameters on antifungal peptides from Supermeal worm, Zophobas morio (Fabricius)

Faruck, Mohammad Omer and Yusof, Faridah and Chowdhury, Md. Sazzad Hossien (2017) Effect of extraction process parameters on antifungal peptides from Supermeal worm, Zophobas morio (Fabricius). International Food Research Journal (IFRJ), 24 (Suppl.). pp. 463-467. ISSN 1985-4668 E-ISSN 2231-7546

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Abstract

Acidified isopropanol extract of whole body larvae of Zophobas morio (Fabricius), which contains peptides, has been shown to exhibit an inhibitory effect towards fungal growth. The larvae, commonly known as supermeal worm are cheap and easily maintained. To make the extraction even more cost effective, it is pertinent to maximize the extraction yield and to optimize the extraction process. The aim of this study is to use the One-Factor-At-a-Time (OFAT) strategy to determine the maximum values of the process parameters for the extraction of antifungal peptides, where these values can later be used in the experimental design to optimize the extraction process. Based on importance, three parameters were selected, namely, initial homogenization temperature, homogenization time and solid (g) to solvent (ml) ratio. Maximum inhibition to fungal growth was found when the extraction was carried out as follows; using initial homogenization temperature of 4°C, homogenization time of 5 minutes and a solid (g) to solvent (ml) ratio of 3.5:1. The peptide extract displayed different degree of antifungal effect towards four selected fungi, Aspergillus niger, Microsporum canis, Candida albicans and Blastomyces dermatitidsis.

Item Type:	Article (Journal)
Additional Information:	4734/59631
Uncontrolled Keywords:	Peptides, antifungal, One-Factor-At-a-Time (OFAT), Zophobas morio, Extraction
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	DR FARIDAH YUSOF
Date Deposited:	24 Nov 2017 11:54
Last Modified:	19 Apr 2018 16:20
URI:	http://irep.iium.edu.my/id/eprint/59631

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