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Anticancer peptides derived from supermeal worm (Zophobas morio) larvae

Yusof, Faridah and Chowdury, Silvia and Faruck, Mohammad Omer and Sulaiman, Nadzril (2017) Anticancer peptides derived from supermeal worm (Zophobas morio) larvae. International Food Research Journal (IFRJ), 24. S456-S451. ISSN 2231-7546

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Abstract

Cancer still presents enormous challenges in the medical world. Currently, the search for anticancer compounds has garnered a lot of interest, especially in finding them from the natural sources. In this study, by using Sulforhodamine B (SRB) colorimetric assay, compounds, extracted from supermeal worm (Zophobas morio) larvae using two types of acidified organic solvent (ethanol and isopropanol), were shown to inhibit the growth of a breast cancer line, MCF-7. A comparative study of the effect was carried out on a normal cell line, Vero. Results showed that, the two types of extracts inhibits growth of MCF-7 cell at varying degrees, on the other hand, have much less effect on Vero cell. Extracts analysed by UV-vis spectroscopy, showed peaks in the range of 260 to 280 nm, inferring the presence of aromatic amino acids, whereas the highest peak of 3.608 AU at 230 nm indicates the presence of peptide bonds. By Raman spectroscopy, peaks are observed at 1349 cm-1, 944 cm-1 and 841 cm-1 indicating the presence of Tyr, Try and Gly, confirming the UV-vis analyses. All results of analyses implied that the anticancer compounds contain peptides.

Item Type: Article (Journal)
Additional Information: 4734/59628
Uncontrolled Keywords: Anticancer peptides, Zophobas morio Fabricus, MCF-7 breast cancer cell line, Vero cell line.
Subjects: T Technology > TP Chemical technology > TP155 Chemical engineering
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Kulliyyah of Engineering > Department of Mechatronics Engineering
Depositing User: DR FARIDAH YUSOF
Date Deposited: 28 Nov 2017 14:08
Last Modified: 25 Jun 2018 11:57
URI: http://irep.iium.edu.my/id/eprint/59628

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