Enzyme-aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed

Hammed, Ademola Monsur and Jaswir, Irwandi and Senay, Simsek and Alam, Md Zahangir and Amid, Azura (2017) Enzyme-aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed. International Food Research Journal, 24 (4). pp. 1660-1666. ISSN 1985 4668 E-ISSN 2231 7546

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Abstract

This study involves extraction of sulfated polysaccahride (SP) from brown seaweed (Turbinaria turbinata). Eight processing conditions affecting enzyme aided extraction (EAE) were screened using Plackett-Burman design. Three signifcant factors (hydrolysis time, enzyme concentration and extraction stage) were optimized using Faced Centred Central Composite Design in Random Surface Methods. Micrograph obtained using Field Emission Scanning Electron Microscopy revealed that cellulase degradation ruptured the seaweed cell matrix thus caused an increase in the release of SP. The optimum conditions for extraction of SP from T. turbinata are: extraction stage of 2, hydrolysis time of 19.5 h and enzyme concentration of 1.5 μl/ml to produce 25.13% yield. The SP obtained from cellulase treated T. turbinata is a suitable anti-inflammatory agent for pharmaceutical applications.

Item Type:	Article (Journal)
Additional Information:	7662/58457
Uncontrolled Keywords:	Sulfated-polysaccharide, Enzyme-aided-extraction, Brown seaweed, optimization, Fucoidan
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof Ts Dr Azura Amid
Date Deposited:	25 Sep 2017 11:35
Last Modified:	20 Mar 2018 13:11
URI:	http://irep.iium.edu.my/id/eprint/58457

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