Investigation of anticancer peptides from zophobas moria fabricus

Chowdhury, Silvia and Yusof, Faridah and Faruck, Mohammad Omer and Sulaiman, Nadzril (2015) Investigation of anticancer peptides from zophobas moria fabricus. In: Asian Congress on Biotechnology 2015 (ACB 2015), 15th-19th Nov 2015, Istana Hotel, Kuala Lumpur. (Unpublished)

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Abstract

Cancer is an extremely complicated disease and still presents enormous challenges, in the medical world. Drug-based chemotherapy treatment are usually applied especially to combat cancer that cannot be treated by any other cancer treating method. Up-to-date there are so many drugs been researched and discovered and some of them are at the different phases of trials before commercialization. Anticancer agents based on natural resources are preferred compared to chemical based because the later can also be harmful towards healthy cells, thus elevating the side effects of chemotherapy. In this study, anticancer peptides has been recovered from non-immunized final instar larvae of Zophobas morio Fabricus using acidified (triflouroacetic acid) solvent (ethanol and isopropanol). Results showed that the two different extracts inhibits growth of MCF-7 breast cancer cell line at varying degrees. The results of cytotoxicity investigation and fluorescence microscopic observations conclude that the extracts could significantly inhibit MCF-7 cell viability and disrupt their cell morphology. Statistical analysis showed ethanolic and isopropanolic extract have IC50 of 1.7 mg/ml and 0.7 mg/ml respectively. This work may present the first initiative towards recovering anticancer peptides from natural, low cost, easily found insect larvae, whereby it can be potentially developed as peptide-based drug to be used in chemotherapy. Future work includes purifying, identification and characterization of peptides.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	4734/55092
Uncontrolled Keywords:	zophobas morio fabricus, MCF-7 breast cancer cancer cell line, chemotherapy, cytotoxicity, fluorescence microscopy
Subjects:	T Technology > T Technology (General) T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	DR FARIDAH YUSOF
Date Deposited:	30 Mar 2017 10:30
Last Modified:	30 Mar 2017 10:30
URI:	http://irep.iium.edu.my/id/eprint/55092

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