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Effects of ionizing radiation on chondrocytes growth: work in progress

Mat Nawi, Nur Farhana and Sha'ban, Munirah and Zainuddin, Zainul Ibrahim (2016) Effects of ionizing radiation on chondrocytes growth: work in progress. In: 6th MTERMS 2016 in conjunction with 2nd Malaysian Stem Cells Meeting, 17 - 18 Nov 2016, Penang. (Unpublished)

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Abstract

Purpose: To evaluate the possible effects of ionizing radiation on cellular morphology and growth kinetics of monolayer cultured chondrocytes. Methods: Following research approval (IIUM/IACUC Approval/2015/[5][24]), rabbit’s articular cartilage cells were isolated, cultured and divided into three groups; (1) control, (2) irradiated and (3) non-irradiated. Group 2 cells were subjected to irradiation after it reaches 80-90% confluency. While the control group was left in the incubator, groups 2 and 3 were taken out and put into a temperature-controlled container. They were transported to a typical X-ray examination room, some distance away from the incubator. After irradiating Group 2, both cells groups were returned into the incubator. The X-ray imaging parameters and the collimation used were recorded. Group 2 cells were subjected to irradiation at each different passage, from P0 till P3. Morphological observation and growth kinetics assessments were conducted on all groups after each passage. Results: There are no differences in terms of cellular morphology in all groups throughout passages. The viability of all groups ranged from 94.1-98.6%. All groups showed reduction in growth rate after several passages. The non-irradiated group exhibited higher total cell yield than the other groups. The performance of growth kinetics of all groups can be appreciated as follows; non-irradiated group > irradiated > control. Conclusion: Ionizing radiation may have certain effects on the growth of chondrocytes.

Item Type: Conference or Workshop Item (Slide Presentation)
Additional Information: 6006/53947
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User: Zainul Ibrahim Zainuddin
Date Deposited: 26 Dec 2016 12:38
Last Modified: 26 Dec 2016 12:38
URI: http://irep.iium.edu.my/id/eprint/53947

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