Optimisation of supercritical fluid extraction of astaxanthin from penaeus monodon waste using ethanol-modified carbon dioxide

Azfar Radzali, Shazana and Markom, Masturah and Baharin, Badlishah Shah and Othman, Rashidi and Abdul Rahman, Russly (2016) Optimisation of supercritical fluid extraction of astaxanthin from penaeus monodon waste using ethanol-modified carbon dioxide. Journal of Engineering Science and Technology, 11 (5). pp. 722-736. ISSN 1823-4690

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Abstract

Some studies demonstrated that astaxanthin surpasses the antioxidant benefits of beta-carotene, zeaxanthin, canthaxanthin, vitamin C, and vitamin E. Penaeus monodon (Tiger shrimp) is one of the most valuable traded crustacean products in which astaxanthin can be found in its by-products. The extraction of thermolabile compound like carotenoids at lower temperatures through supercritical carbon dioxide (SC-CO2) can reduce the potential isomerization and degradation of the extraction product. In this study, astaxanthin had been extracted using SC-CO2 with 15% (v/v) ethanol as an entrainer and the recovered astaxanthin was analyzed using High performance liquid chromatography (HPLC). A central composite design (CCD) was employed to study the effect of three SC-CO2 parameters namely temperature (X1) from 40 to 80°C, pressure (X2) from 150 to 250 bar and extraction flow rate (X3) from 1 to 3 ml/min on the astaxanthin complex yield, (Y1) and free astaxanthin content, (Y2). The nonlinear regression equations were significantly (p<0.05) fitted for both responses with high R2 (>0.9261), which had no indication of lack of fit. The results indicated that a combined set of values of temperature (56.88°C), pressure (215.68 bar) and extraction flow rate (1.89 ml/min) was predicted to provide the optimum region in terms of astaxanthin complex yield, (58.50 ± 2.62 μg/g ) and free astaxanthin content (12.20 ± 4.16 μg/g) studied.

Item Type:	Article (Journal)
Additional Information:	3842/53555
Uncontrolled Keywords:	Astaxanthin, carotenoids, HPLC, optimization, penaeus monodon, SFE
Subjects:	Q Science > QD Chemistry S Agriculture > SH Aquaculture. Fisheries. Angling T Technology > TP Chemical technology T Technology > TP Chemical technology > TP248.13 Biotechnology T Technology > TP Chemical technology > TP368 Food processing and manufacture T Technology > TP Chemical technology > TP934 Paints, pigments, varnishes, etc
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Architecture and Environmental Design > Department of Landscape Architecture
Depositing User:	Dr rashidi othman
Date Deposited:	21 Dec 2016 09:05
Last Modified:	03 Jan 2017 14:34
URI:	http://irep.iium.edu.my/id/eprint/53555

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