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Bromelain enzyme assay using Casen as substrate

Amid, Azura and Jamaluddin, Mohd Jamil Aizat (2016) Bromelain enzyme assay using Casen as substrate. In: Experimental methods in modern biotechnology Volume 2. IIUM Press Series of Textbook from the Islamic Perspectives, 2 . IIUM Press, Kuala Lumpur, pp. 100-150. ISBN 978-967-418-384-4

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Abstract

This procedure is following Sigma's non-specific protease activity. In this assay, casein acts as a substrate to the reaction. When protease digest casein, the amino acid tyrosine is liberated along with other amino acids and peptide fragments. Folin & Ciocalteus Phenol, or Folin's reagent primarily reacts with free tyrosine to produce a blue colored chromophore, which is quantifiable and measured as an absorbance value on the spectrophotometer at 660 nm absorbance. The more tyrosine that is released from the casein, the more chromophores are generated and the stronger the activity of the protease.

Item Type: Book Chapter
Additional Information: 3688/49849
Uncontrolled Keywords: bromelain, enzyme, asssay. cascin substrate
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Azura Amid
Date Deposited: 21 Jul 2016 16:16
Last Modified: 21 Feb 2017 10:05
URI: http://irep.iium.edu.my/id/eprint/49849

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