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Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase

Jimat, Dzun Noraini and Mohamed , Intan Baizura Firda (2016) Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase. In: Experimental Methods in Modern Biotechnology. IIUM Press Series of Textbook from the Islamic Perspectives, 2 . IIUM Press, Kuala Lumpur, pp. 72-80. ISBN 978-967-418-384-4

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This procedure is following Gulati, Saxena, and Gupta (1997) protocol which is known as semi-quantitative plate assay for screening L-asparaginase- producing bacterial from local hot spring. The plate agar medium containing asparagine (as sole nitrogen sourse) is prepared with addition of phenol red which later shows an indication of L-asparaginase based on changes of colour. Phenol red at acidic is yellow which might change colour to pink when it is at alkaline. Thus, it indicates the formation of pink zone around microbial colonies producing L-asparaginase. The inoculated agar plates are incubated at 370C in an incubator for 24 hours. Thus, the isolation and screening of bacterial producing L-asparaginase are based on phenol-red zone. After purification, the isolates are characterised using several biochemical tests.

Item Type: Book Chapter
Additional Information: 3891/49762
Uncontrolled Keywords: isolation, characterisation, thermophilic, bacterial, L-asparaginase
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Dzun Noraini Jimat
Date Deposited: 17 Apr 2016 18:21
Last Modified: 16 Jan 2017 14:23
URI: http://irep.iium.edu.my/id/eprint/49762

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