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Protein purification in chromatographic media using multiwall carbon nanotubes

NM, Mubarak and Yusof, Faridah (2015) Protein purification in chromatographic media using multiwall carbon nanotubes. Journal of Bioprocessing & Biotechniques, 5 (3). p. 1000214. ISSN 2155-9821

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Abstract

Carbon Nanotubes (CNTs) had been synthesized by Double Stage Chemical Vapor Deposition (DS-CVD) at the gas phase with Hydrogen gas (H2) and acetylene (C2H2) as precursor gases. Moreover, the structure of CNTs, morphology and purity were characterized by using the Transmission Electron Microscope (TEM), Field Emission Scanning Electron Microscope (FESEM) and Thermogravimetric Analysis (TGA). Furthermore, the best conditions for the optimal CNT was reaction at temperature of 750°C, reaction time of 30 minutes, with gas flow rates H2 and C2H2 for 60 and 170 ml/min for respectively. The hydrochloric acid was used to purify produced CNT and then using the nitric acid and sulfuric treatment to functionalize. Moreover, functionalized CNTs, non-functionalized were used as column chromatographic media for skim latex protein purification. Thus, the results reveal that at pH7 and ionic strength 50 mM gives higher efficiency of protein removal from the skim latex.

Item Type: Article (Journal)
Additional Information: 4734/44453
Uncontrolled Keywords: CNTs; DS-CVD; Functionalization; Skim latex serum; Protein purification
Subjects: T Technology > TP Chemical technology > TP155 Chemical engineering
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: DR FARIDAH YUSOF
Date Deposited: 27 Aug 2015 09:45
Last Modified: 10 Nov 2017 16:42
URI: http://irep.iium.edu.my/id/eprint/44453

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