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Case Study: Recombinant Bromelain Downstream Processing

Amid, Azura and Mohd Arshad, Zatul Iffah and Othman, Muhd. Ezza Faiez (2015) Case Study: Recombinant Bromelain Downstream Processing. In: Recombinant Enzymes—From Basic Science to Commercialization. Springer International Publishing, Cham Heidelberg New York Dordrecht London, pp. 175-185. ISBN 978-3-319-12396-7

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Abstract

This chapter presents details on the purification, formulation and drying of recombinant bromelain. The wide range of applications of recombinant bromelain has increased interest in finding viable purification techniques for large-scale production. An affinity chromatography technique was developed by Amid and co-workers (Expression, purification, and characterization of a recombinant stem bromelain from Ananas comosus, Process Biochem 46:2232–2239, 2011) to purify recombinant bromelain. However, this technique presented low recovery and small sample loading capacity and thus is not suitable as a purification tool in the largescale production of recombinant bromelain. An aqueous two-phase system is one alternative method that we use to purify recombinant bromelain, as it reliable and easy to scale up and has a low cost. As part of avoiding cysteine degradation, spray drying the purified recombinant protein with maltodextrin as an excipient provides the possibility of preserving its activity and creating fine particles that are suitable for end-product application. The processes of the purification, formulation and spray drying of recombinant bromelain are explained briefly.

Item Type: Book Chapter
Additional Information: 3688/43384
Uncontrolled Keywords: back extraction, continous ultrasonication, Gibbs free energy, inlet air temperature, PEG-rich phase, salting-out, immiscible
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Azura Amid
Date Deposited: 13 Jun 2015 00:07
Last Modified: 31 Jul 2015 10:41
URI: http://irep.iium.edu.my/id/eprint/43384

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