Effects of operating conditions in spray drying of recombinant bromelain

Amid, Azura and Othman, Muhd. Ezza Faiez and Jamaluddin, Mohd Jamil Aizat (2014) Effects of operating conditions in spray drying of recombinant bromelain. Journal of Applied Science and Agriculture, 9 (20). pp. 37-43. ISSN 1816-9112

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Abstract

The aim of the present study is to investigate the effect of operating parameter of a Buchi B290 mini spray dryer towards specific activity of recBromelain. Using Response Surface Methodology (RSM), face centred central composite design (FCCCD) was tabulated for optimization. Optimization process was conducted by varying the inlet air temperature (°C), gas flow height (mm) and pump settings (%) of the laboratory Buchi B290 Mini Spray Dryer to obtained optimal spray drying operating processes recovering high recBromelain specific activity as response. Results showed that the optimized process parameter having 126 °C air inlet temperature, 42 mm gas flow height and 12 % feed pump setting resulted in 0.119 ± 0.003 U/mgprotein of spray dried recBromelain with R2 of 90.12% thus this model was found to be significance and reliable.

Item Type:	Article (Journal)
Additional Information:	3688/39951
Uncontrolled Keywords:	Bromelain, Recombinant Response Surface Methodolody Spray Dry
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof Ts Dr Azura Amid
Date Deposited:	17 Dec 2014 21:27
Last Modified:	24 May 2018 09:33
URI:	http://irep.iium.edu.my/id/eprint/39951

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