Apoptotic activities of thymoquinone, an active ingredient of black seed (nigella sativa), in cervical cancer cell lines

Arief Ichwan, Solachuddin Jauhari and Al-Ani, Imad Matloub Dally and Bilal, Hakim Gharib and Suriyah, Wastuti Hidayati and Bakhtiar, M. Taher and Ikeda, Masa-Aki and Bilal, Hakim Gharib (2014) Apoptotic activities of thymoquinone, an active ingredient of black seed (nigella sativa), in cervical cancer cell lines. Chinese Journal of Physiology , 57 (5). 249 -255. ISSN 0304-4920

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Abstract

Thymoquinone (TQ) is the main constituent of black seed (Nigella sativa, spp) essential oil which shows promising in vitro and in vivo anti-neoplastic activities in different tumor cell lines. However, to date there are only a few reports regarding the apoptotic effects of TQ on cervical cancer cells. Here, we report that TQ stimulated distinct apoptotic pathways in two human cervical cell lines, Siha and C33A. TQ markedly induced apoptosis as demonstrated by cell cycle analysis in both cell lines. More- over, quantitative PCR revealed that TQ induced apoptosis in Siha cells through p53-dependent pathway as shown by elevated level of p53-mediated apoptosis target genes, whereas apoptosis in C33A cells was mainly associated with the activation of caspase-3. These results support previous findings on TQ as a potential therapeutic agent for human cervical cancer.

Item Type:	Article (Journal)
Additional Information:	5731/38368
Uncontrolled Keywords:	apoptosis, caspase, cervical cancer, Nigella sativa, thymoquinone
Subjects:	R Medicine > RB Pathology R Medicine > RM Therapeutics. Pharmacology > RM300 Drugs and their action
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry Kulliyyah of Medicine > Department of Basic Medical Kulliyyah of Pharmacy
Depositing User:	Dr. Solachuddin J.A. Ichwan
Date Deposited:	24 Sep 2014 13:11
Last Modified:	12 Jun 2018 10:04
URI:	http://irep.iium.edu.my/id/eprint/38368

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