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Purification and cytotoxicity assay of tomato (Lycopersicon Esculentum) leaves methanol extract as potential anti-cancer agent

Wan Chik, Wan Dalila and Amid, Azura and Jamal, Parveen (2010) Purification and cytotoxicity assay of tomato (Lycopersicon Esculentum) leaves methanol extract as potential anti-cancer agent. In: 2010 International Conference on Process Engineering and Advanced Materials (icpeam2010), 15th-17th June 2010, Kuala Lumpur Convention Centre, Kuala Lumpur.

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Abstract

This research studied the cytotoxicity effect of tomato leaves methanol extract on cancer cells to address potential therapeutic in MCF-7 breast cancer cell lines and its toxicity towards Vero cells. The extraction was done in a shake flask by 82% methanol, 1:10 (w/v), agitated at 22 °C with 110 rpm within 24 hours. Later, purification process was started by thin layer chromatography (TLC) subjected to determine the best mobile phase for compound separation and collection by means of column chromatography. Next, the effect of purified sample towards MCF-7 breast cancer cell lines and Vero cells were observed using in vitro cytotoxicity assay to indicate its active fractions and its half maximal inhibitory concentration (IC50). Purified sample gave a rational effect towards MCF-7 breast cancer cells with IC50 value of 5.85 μg/ml compared to Taxol with IC50 value of 0.039 μg/ml. The purified sample can also be judged to be harmless as it has IC50 value of 765.6 μg/ml in Vero cells treatment while Taxol gave IC50 value of 0.045 μg/ ml.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 3688/30512
Uncontrolled Keywords: tomato (Lycopersicon Esculentum)
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Azura Amid
Date Deposited: 21 Jun 2013 11:19
Last Modified: 25 Jun 2013 09:38
URI: http://irep.iium.edu.my/id/eprint/30512

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