Analysis of fucoxanthin content and purification of all-trans-fucoxanthin from turbinaria turbinata and sargassum plagyophyllum by SiO2 open column chromatography and reversed phase-HPLC

Jaswir, Irwandi and Noviendri, Dedi and Mohd. Salleh, Hamzah and Bakhtiar, M. Taher and Miyashita, Kazuo and M. Ramli, Nazaruddin (2013) Analysis of fucoxanthin content and purification of all-trans-fucoxanthin from turbinaria turbinata and sargassum plagyophyllum by SiO2 open column chromatography and reversed phase-HPLC. Journal of Liquid Chromatography & Related Technologies, 36 (10). pp. 1340-1354. ISSN 1520-572X (O), 1082-6076 (P)

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Abstract

Reversed phase-high performance liquid chromatography (RP-HPLC) was used to analyze fucoxanthin content of two species of Malaysian brown seaweeds, Turbinaria turbinate andSargassum plagyophyllum. The fucoxanthin contents of T. turbinata, and S. plagyophyllum were 0.59 ± 0.08 and 0.71 ± 0.01 mg/g dry-weight, respectively. Ultraviolet spectrum of fucoxanthin showed the maximum absorbance at 450 nm. Furthermore, the HPLC chromatograms of purified all-trans-fucoxanthin from showed one major peak with a retention time of 7.8 minutes.

Item Type:	Article (Journal)
Additional Information:	5784/28716
Uncontrolled Keywords:	extraction; fucoxanthin; open column chromatography; purification; reversed phase-HPLC; Sargassum plagyophyllum; thin layer chromatography; Turbinaria turbinata
Subjects:	Q Science > QD Chemistry T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof. Dr. Irwandi Jaswir
Date Deposited:	03 Oct 2013 15:15
Last Modified:	14 Jul 2021 17:51
URI:	http://irep.iium.edu.my/id/eprint/28716

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