Optimization of extraction process parameters for anti-cancer agent from Solanum Lycopersicum

Amid, Azura and Ahmad, Abdul Aziz and Ahmad Raus, Raha (2011) Optimization of extraction process parameters for anti-cancer agent from Solanum Lycopersicum. In: Current research and development in biotechnology engineering at IIUM. IIUM Press, Kuala Lumpur, pp. 1-9. ISBN 9789674181444

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Abstract

In the fight against cancer, novel chemotherapeutic agents are continually being sought to complement existing drugs. The optimize extraction process is required to ensure that the maximum yield of anti-cancer agents will be extracted. In this study, three extraction parameters were investigated on their effects toward the anti-cancer activities when applied to MCF-7 breast cancer cell line. A three-factor and three levels Box Behnken design consisting of seventeen experiments was employed including five replicates at the center point. The response variable will be the absorbance reading at 490 nm in terms of percentage cell viability. The design variables were the extraction time (min), extraction agitation speed (rpm) and solvent composition (%, v/v water/methanol). The optimum extraction was found to be 82% methanol, 24 hours and 110 rpm.

Item Type:	Book Chapter
Additional Information:	3688/24484
Uncontrolled Keywords:	Box Behnken design; design ofexperiment; Solanum lycopersicum
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof Ts Dr Azura Amid
Date Deposited:	03 Jul 2012 14:12
Last Modified:	03 Jul 2012 14:13
URI:	http://irep.iium.edu.my/id/eprint/24484

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