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Investigations on spider house for anti microbial activity

Mirghani, Mohamed Elwathig Saeed and Zulkifli, Mohamad Zul Fahmi (2011) Investigations on spider house for anti microbial activity. In: Current research and development in biotechnology engineering at IIUM. IIUM Press, Kuala Lumpur, pp. 117-128. ISBN 9789674181444

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The purpose of this new investigation was to determine if spider webs exhibit antibacterial properties. In order to determine antibacterial properties, the spider webs were extracted with different solvents such as methanol, ethanol, acetone, and water in different conditions (extraction time, and concentration used for optimization). These extracts were screened for antibacterial activity using disc diffusion assay. Two bacteria were used in the antibacterial assay namely Bacillus subrilis, and Escherichia coli The determination of spider webs exhibiting antibacterial properties was based, at least in this study, solely upon the definite appearance of an inhibition zone around the well of plates. In screening, acetone solvent was shown the best antibacterial activity compare to other solvents with 10 mm of diameter of inhibition zone for Bacillus subtilis and 9 mm of diameter of inhibition zone for Escherichia coli. In optimization, the maximum inhibition zone on the Bacillus subtilis was 15 rom at a time of 48 hours and concentration of 0.035 g/ml. Meanwhile, the maximum diameter of inhibition zone on the Escherichia coli was 12 mm at a time of 48 hours and concentration of 0.035 g/ml. Therefore, this study showed that spider webs could be potential source of new antibacterial agents.

Item Type: Book Chapter
Additional Information: 4971/20959
Uncontrolled Keywords: heat resistance, chocolate
Subjects: T Technology > TP Chemical technology > TP368 Food processing and manufacture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: A Prof Elwathig Mirghani
Date Deposited: 04 Jul 2012 09:03
Last Modified: 04 Jul 2012 09:03
URI: http://irep.iium.edu.my/id/eprint/20959

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