Synbiotic Smallanthus Sonchifolius (Yacon) and Streptococcus Salivarius Inhibit Candida Albicans biofilm formation

Rismayuddin, Nurul Alia Risma and Syed Nasarudin, Syarifah Nurhikmah Izzati and Ibrahim, Nurul Ruziantee and Alnuaimi, Ali and Arzmi, Mohd Hafiz (2023) Synbiotic Smallanthus Sonchifolius (Yacon) and Streptococcus Salivarius Inhibit Candida Albicans biofilm formation. In: International Conference on Biomedical Engineering (ICoBE 2021), 14- 15 September, 2021, Arau, Perlis.

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Abstract

Oral biofilms are comprised of extracellular polysaccharides and polymicrobial microorganisms. The objective of this study was to determine the effect of synbiotic Smallanthus sonchifolius (yacon) with Streptococcus salivarius K12 (K12) on polymicrobial biofilm formation of Candida albicans with the hypothesis that polymicrobial biofilm biomass of C. albicans is inhibited by synbiotic S. sonchifolius with K12. Initially, disk diffusion and well diffusion assay were conducted to determine the susceptibility of C. albicans towards S. sonchifolius and K12. Following that, C. albicans was mixed with S. salivarius in nutrient broth (NB) or RPMI-1640 to determine the effect of probiotic on the polymicrobial biofilm. To determine the effect of synbiotic, similar protocol was repeated by adding 800 mg mL-1 of S. sonchifolius aqueous extract extract into the same followed by a 72 h incubation. Finally, biofilm biomass was measured using a crystal violet assay. C. albicans ATCC MYA-4901, ALC2 and ALC3 were found to be susceptible to S. sonchifolius extract and S. salivarius K12. However, the biofilm of all of C. albicans strains ATCC MYA-4901, ALC2 and ALC3 were found to reduce ranged in between 20% to 39.4% when co-cultured with synbiotic compared to prebiotic culture in NB. In conclusion, synbiotic S. sonchifolius with K12 inhibit polymicrobial biofilm. This indicates the potential use of synbiotic in dental application for the prevention C. albicans infection.

Item Type:	Conference or Workshop Item (Slide Presentation)
Uncontrolled Keywords:	Smallanthus Sonchifolius, Streptococcus Salivarius, Candida albicans, biofilm
Subjects:	Q Science > QR Microbiology R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences Kulliyyah of Dentistry > Department of Oral Maxillofacial Surgery and Oral Diagnosis
Depositing User:	AP Ts Dr Mohd Hafiz Arzmi
Date Deposited:	06 Mar 2023 13:17
Last Modified:	05 Jul 2023 15:38
URI:	http://irep.iium.edu.my/id/eprint/103825

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