The effects of the topical application of eurycoma longifolia jack (TA) root extract hydrogel on vascular endothelial growth factor (VEGF) expression during wound healing in vivo excisional wound model

Al-Bayati, Maryam Riyadh Yaseen and Faisal, Ghasak Ghazi and Abd Fuaat, Azliana and Ahmad Affandi, Khairunisa and Alallam, Batoul (2023) The effects of the topical application of eurycoma longifolia jack (TA) root extract hydrogel on vascular endothelial growth factor (VEGF) expression during wound healing in vivo excisional wound model. IIUM Medical Journal Malaysia, 22 (1). pp. 79-86. E-ISSN 2735-2285

	PDF (Article) - Published Version Restricted to Registered users only Download (1MB) | Request a copy
Preview	PDF (Scopus) - Supplemental Material Download (738kB) | Preview

Abstract

INTRODUCTION: Medicinal plants are known for their positive impacts on wound healing by stimulating angiogenesis in the skin. Eurycoma longifolia Jack locally known as Tongkat Ali (TA), is a medicinal plant characterised by anti-inflammatory and antioxidant effects. MATERIALS AND METHODS: Excisional wound (15mm × 15mm) in diameter and (2mm) depth was created at the back of 20 male Sprague Dawley rats by incising the marked skin with sterilized surgical scalpel blade then excised the skin by surgical scissors and toothed forceps. The wounded rats were divided into 4 groups, each group contained 5 rats (n=5). Experimental groups were formed as follows: untreated (-ve) control, Hydrocyn® aqua gel (+ve) control, vehicle hydrogel, and TA hydrogel. All the treatments were applied twice daily for 5 days starting on the first day (wounding day). On Day 5 post-wounding, the granulation tissue was harvested from all groups and evaluated by immunohistochemistry assay for vascular endothelial growth factor (VEGF) expression. RESULTS: VEGF expression in granulation tissue of rat’s skin treated with TA hydrogel and vehicle hydrogel increased significantly compared to that in the untreated (-ve) control group with p-values of 0.040 and 0.029, respectively. Although there was no significant difference in VEGF expression in granulation tissue of rat’s skin treated with the TA group, Hydrocyn® aqua gel (+ve) control, and vehicle hydrogel groups, our study group showed higher expression of VEGF. CONCLUSION: Our study showed that the topical application of TA hydrogel increased the VEGF expression in granulation tissue of rat’s skin, which is an essential growth factor for wound healing. Thus, there is great potential for TA hydrogel to be an effective wound-healing agent for managing cutaneous wounds.

Item Type:	Article (Journal)
Uncontrolled Keywords:	eurycoma longifolia jack, wound healing, vascular endothelial growth factor, immunohistochemistry
Subjects:	R Medicine > RB Pathology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences Kulliyyah of Medicine Kulliyyah of Medicine > Department of Pathology & Lab Medicine
Depositing User:	Assoc.Prof Ghasak Ghazi Al-Atrakchi
Date Deposited:	09 Jan 2023 10:59
Last Modified:	26 Dec 2023 15:26
URI:	http://irep.iium.edu.my/id/eprint/103075

Actions (login required)

View Item